May 29, 2026
Enter working volume, flow rate, and viable cell density to get perfusion rate (VVD), dilution rate, residence time, cell-specific perfusion rate (CSPR), and media consumption — with a live stirred-tank visualization. A free, no-login continuous-culture calculator that runs in your browser.
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Convert relative centrifugal force (RCF, × g) to and from RPM using your rotor's radius. Pick a rotor from the library or enter a custom radius, type RPM or RCF, and the other updates live — with a spinning-rotor visualization, tip speed, and angular velocity. Runs in your browser.
Calculate doubling time, specific growth rate, and number of doublings from two timepoints or a full time course — or project forward to predict cell counts and time-to-target. Works with cell counts, confluence %, or OD₆₀₀. Runs entirely in your browser, no login.
Enter NA, emission wavelength and immersion medium to get diffraction-limited lateral and axial resolution, plus the pixel size you need to sample it (Nyquist). Add your camera pixel and magnification to check whether you're under- or over-sampling — with a live Airy-disk + sampling-grid visualization. Runs in your browser.
Work out how much to weigh for a target molarity, do C1V1 = C2V2 dilutions, and lay out serial dilutions — pick what to solve for and everything updates live, with unit selectors and a beaker visualization. Runs in your browser.
Design 6–1536-well plate layouts, lay out dilution series, paste your results for a heatmap, and fit dose-response curves (EC₅₀/IC₅₀) — all in the browser. Export PNG, SVG, CSV, or JSON. No login.
Paste a protein sequence to get molecular weight, theoretical pI, molar extinction coefficient (280 nm), GRAVY hydropathy, aliphatic index, charge, and amino-acid composition — with a net-charge-vs-pH curve. A free, no-login ProtParam-style tool that runs in your browser.
Turn qPCR Ct values into relative expression with the Livak ΔΔCt method. Enter target and reference (housekeeping) Ct per sample, mark the calibrator, and get ΔCt, ΔΔCt and 2^−ΔΔCt fold change — with a fold-change bar chart. Replicate Cts are averaged. Runs in your browser.
Calculate a primer's melting temperature with the nearest-neighbor model (SantaLucia 1998, salt-corrected) and map restriction sites across the common cloning enzymes — both strands, linear or circular. Runs entirely in your browser, no login.
May 25, 2026
Paste a CDS or protein, pick a host organism + real constraints (avoid restriction sites, GC bounds, no homopolymers), and get an optimized DNA sequence ready for synthesis ordering. No login required.
A free, embeddable 3D molecular structure viewer for any PDB entry or AlphaFold prediction — powered by Mol*, the same engine used by RCSB PDB, PDBe, and the AlphaFold Database.
Paste or upload a GenBank file, get a publication-quality plasmid map (linear or circular) you can download as SVG. Powered by the open-source DNA Features Viewer.
November 30, 2025
An actively maintained, searchable database of open-source tools for biological cellular image and time-lapse analysis — ranked by GitHub stars, recent publications, and historical popularity.
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