Advancing tissue engineering through a portable perfusion and incubation system
Summary
Perfusion enhances engineered tissues by improving nutrient and oxygen delivery. This research presents a lightweight (< 10 lb) portable perfusion system — FluidON — combined with the CultureON CO₂ cell culture incubator, capable of maintaining continuous flow under standard incubation conditions.
The device generates physiologically relevant interstitial flow rates and ensures uniform nutrient distribution, confirmed by computational modeling. When tested with bioengineered tissue patches, the system maintained cell viability, improved spatial organization, strengthened the tissue matrix, and slowed hydrogel degradation. The system enables mobile perfusion and incubation for advancing tissue maturation and bioengineering research.
Full article
Read the full paper on Bioengineering (MDPI) →
Published in the special issue The New Frontiers of Artificial Organs Engineering.
Leading organizations
University of Chicago (Dr. Narutoshi Hibino laboratory) and 37degrees, Inc.