#delta delta Ct

Interactive tools • May 29, 2026

qPCR ΔΔCt Calculator: relative gene expression (fold change) from Ct values

Turn qPCR Ct values into relative expression with the Livak ΔΔCt method. Enter target and reference (housekeeping) Ct per sample, mark the calibrator, and get ΔCt, ΔΔCt and 2^−ΔΔCt fold change — with a fold-change bar chart. Replicate Cts are averaged. Runs in your browser.

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