#numerical aperture

Interactive tools • May 29, 2026

Microscopy Resolution & Nyquist Calculator: optimal pixel size from NA & wavelength

Enter NA, emission wavelength and immersion medium to get diffraction-limited lateral and axial resolution, plus the pixel size you need to sample it (Nyquist). Add your camera pixel and magnification to check whether you're under- or over-sampling — with a live Airy-disk + sampling-grid visualization. Runs in your browser.

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